Anti-ZO1 Rabbit monoclonal TJP1 Antibody

Product Name

Anti-ZO1 Rabbit monoclonal TJP1 Antibody

View all Tight Junction Protein 1 Antibodies

SKU/Catalog Number

M00860-1

Size

100ul

Form

Liquid

Description

Boster Bio Anti-ZO1 Rabbit monoclonal TJP1 Antibody catalog # M00860-1. Tested in ICC,IHC applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ZO1 Rabbit monoclonal TJP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00860-1)

Host

Mouse

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Monoclonal

Clone Number

2F2

Isotype

IgG

Immunogen

Synthesized peptide derived from human TGF β Receptor I

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

M00860-1 is reactive to TJP1 in Human, Mouse, Rat

Applications

M00860-1 is guaranteed for IHC, ICC Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

195.459kDa

Background of Tight Junction Protein 1

TRPC6, also known as TRP6, short transient receptor potential channel 6 and transient receptor potential cation channel subfamily C member 6, is thought to form a receptor-activated non-selective calcium permeant cation channel. TRPC6 is probably operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. It is activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C and may not to be activated by intracellular calcium store depletion. Defects in this gene are a cause of focal segmental glomerulosclerosis (FSGS). Expression of this protein has been reported in tissues such as placenta, lung, spleen, ovary, small intestine, and renal podocytes. Immunohistochemistry studies using polyclonal antibodies to this target have shown moderate to strong staining in cell types such as neurons, breast, respiratory, squamous and prostate epithelium, epidermis, placental trophoblasts, dendritic cells, and subsets of immune cells, and faint to moderate staining of adrenal, colon, ganglion cells, hepatocytes, heart, and testis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ICC: 1:200
IHC: 1:200

Validation Images & Assay Conditions

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Figure 2. Immunocytochemistry staining of TJP1 using Anti-ZO1 Rabbit monoclonal TJP1 Antibody (M00860-1).

ICC staining ZO1 in MCF-7 cells (green). Cells were fixed in paraformaldehyde

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Figure 1. Immunohistochemistry validation of TJP1 using Anti-ZO1 Rabbit monoclonal TJP1 Antibody (M00860-1).

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ZO1 antibody. Counter stained with hematoxylin.
For more protocol information of IHC

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Figure 3. Immunohistochemistry validation of TJP1 using Anti-ZO1 Rabbit monoclonal TJP1 Antibody (M00860-1).

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ZO1 antibody. Counter stained with hematoxylin.
For more protocol information of IHC

Gene/Protein Information For TJP1 (Source: Uniprot.org, NCBI)

Alternative Names

DKFZp686M05161; MGC133289; tight junction protein 1 (zona occludens 1); Tight junction protein 1; tight junction protein ZO-1; TJP1; ZO1; ZO-1; zona occludens 1; Zona occludens protein 1; zonula occludens 1 protein; Zonula occludens protein 1 TJP1 ZO-1 tight junction protein 1 tight junction protein ZO-1|zona occludens 1|zonula occludens 1 protein

For more info on TJP1, check out the TJP1 Infographic

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In this infographic, you will see the following information for TJP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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